abstract：:A novel and rapid EBNA-1 titration method has been developed which uses immunoprecipitation of specific DNA-protein complexes with EBNA-1-positive serum. The method is more sensitive than the conventional immunofluorescence method and has potential value as a diagnostic reagent for clinical laboratories. TPA induction...

journal_title：Journal of virological methods

authors： Nonoyama M,Wen LT,Tabata T,Tanaka A

更新日期：1988-09-01 00:00:00

abstract：:All human herpesviruses cause chronic infections in which latent virus is periodically reactivated. Persistently active infections are uncommon, however, and occur exclusively in individuals whose immune systems fail to control virus multiplication and spread. This paper summarizes the management of these unusual infe...

journal_title：Journal of virological methods

authors： Straus SE

更新日期：1988-09-01 00:00:00

abstract：:Systematic solid-phase synthesis of all possible overlapping nonapeptides of the 1381 amino acid sequence of the Epstein-Barr virus major capsid protein (EBV-MCP) was used to identify the position of linear antigen epitopes on this protein as recognised by human polyclonal antisera. Antisera were selected for reactivi...

journal_title：Journal of virological methods

authors： Middeldorp JM,Meloen RH

更新日期：1988-09-01 00:00:00

abstract：:A technique for coupling foot-and-mouth disease virus (FMDV) to tanned sheep red blood cells (SRBC) is reported. Different parameters influencing the procedure were studied. Subtypes C2, C3, O1 and A24 were used as antigens, and guinea pig hyperimmune sera obtained were tested for specific antibody in passive hemagglu...

journal_title：Journal of virological methods

authors： Pujol CA,Feledi CA,Massouh EJ

更新日期：1988-08-01 00:00:00

abstract：:In vitro enzymatic amplification was applied to detect hepatitis B virus (HBV) DNA sequences in serum. This technique, known as the polymerase chain reaction (PCR) was used to amplify a 128 bp DNA fragment including a 112 nucleotide long sequence complementary to a region in the S gene of the HBV genome. Amplified sam...

journal_title：Journal of virological methods

authors： Larzul D,Guigue F,Sninsky JJ,Mack DH,Bréchot C,Guesdon JL

更新日期：1988-07-01 00:00:00

abstract：:A microtiter cell-culture assay is described for measuring neutralizing antibody activity directed against the human immunodeficiency virus (HIV). The assay relies upon inhibition of HIV-mediated cell killing of infected MT-4 lymphoid cells. The assay exhibits comparable sensitivity to two other methods used for such ...

journal_title：Journal of virological methods

authors： Robertson GA,Kostek BM,Schleif WA,Lewis JA,Emini EA

更新日期：1988-07-01 00:00:00

abstract：:Five commercial assays for detecting HIV antigen were evaluated using a panel of 40 coded samples in six laboratories. All assays were capable of detecting HIV-1 antigen (HIV-1 Ag), and are likely to prove useful for monitoring supernatant fluids from a variety of cell cultures. The concentration of HIV-1 protein whic...

journal_title：Journal of virological methods

authors： Healey DS,Maskill WJ,Neate EV,Beaton F,Gust ID

更新日期：1988-06-01 00:00:00

abstract：:Two methods for rapid detection of adenovirus were tested: (i) 24-well plate centrifugation followed by staining with a monoclonal antibody after incubation for 24 h and 48 h, and (ii) pretreatment of A549 cells used in conventional cell culture and 24-well plate centrifugation with 10(-5)M dexamethasone. Twenty-seven...

journal_title：Journal of virological methods

authors： Woods GL,Yamamoto M,Young A

更新日期：1988-06-01 00:00:00

abstract：:530 human faecal samples were examined for the presence of rotavirus by electron microscopy (EM) and by a modified electrophoretic analysis of viral genome (PAGE). 516 stools gave identical results by both methods (97.4% agreement). The proportion of EM+, PAGE- samples (1/530) was significantly lower than the proporti...

journal_title：Journal of virological methods

authors： Pyndiah N,Béguin R,Richard J,Charles M,Rey A,Bonifas V

更新日期：1988-05-01 00:00:00

abstract：:HIV antigen capture assay (ELISA) and reverse transcriptase assay were compared for the detection of HIV. Purified virus and the supernatant of infected cells were used for this study. Antigen capture assay was found to be at least one hundred times more sensitive than the RT assay, and for a large number of samples e...

journal_title：Journal of virological methods

authors： Lee YS

更新日期：1988-05-01 00:00:00

abstract：:In order to evaluate the sensitivity of our modified in situ DNA hybridization technique using biotinylated probes, formalin fixed, paraffin embedded biopsies from 20 cervical lesions known to contain human papillomavirus (HPV) DNA were re-examined by the technique using both 35S-labeled- and biotinylated HPV DNA prob...

journal_title：Journal of virological methods

authors： Syrjänen S,Partanen P,Mäntyjärvi R,Syrjänen K

更新日期：1988-03-01 00:00:00

abstract：:In preparation for field trials, a nuclear polyhedrosis virus (NPV) of the pine beauty moth, Panolis flammea, was mass produced in vivo in an alternative (heterologous) host, the cabbage moth, Mamestra brassicae. Simultaneously, Mamestra NPV was also produced in M. brassicae. This homologous NPV/host system was a cons...

journal_title：Journal of virological methods

authors： Kelly PM,Entwistle PF

更新日期：1988-03-01 00:00:00

abstract：:A logistic ('growth curve') model is formulated and applied to the relationship between numbers of infections (local lesions) produced by a virus on inoculated plants and the concentration of the virus in the inoculum. This model has the advantages of being simple, data-based and therefore not founded on limiting post...

journal_title：Journal of virological methods

authors： Gokhale DV,Bald JG

更新日期：1987-12-01 00:00:00

abstract：:During a 12-month period, two methods for detection of cytomegalovirus (CMV) in 1624 clinical specimens were compared: (1) centrifugal inoculation of MRC-5 cells on coverslips in 24-well plates and staining with a monoclonal antibody to CMV early nuclear antigen after incubation for 40 h (EA assay), and (2) convention...

journal_title：Journal of virological methods

authors： Woods GL,Young A,Johnson A,Thiele GM

更新日期：1987-12-01 00:00:00

abstract：:A solid phase reverse passive hemadsorption test (SP-RPHAd) for hepatitis B surface antigen detection is described. It was compared with a commercial reverse passive hemagglutination assay (Hepatest, Wellcome, U.K.). SP-RPHAd is four-fold less expensive than Hepatest and undiluted sera can be used instead of eight-fol...

journal_title：Journal of virological methods

authors： Duverlie G,Driencourt M,Roussel C,Daniel P

更新日期：1987-11-01 00:00:00

abstract：:Herpes simplex virus (HSV) isolates were differentiated by immunoblotting analysis using a mixture of polyclonal antisera directed against HSV type 1 (HSV-1) and HSV type 2 (HSV-2) glycoprotein fractions (gB/gC of HSV-1 and gC/gE/84-kDa protein of HSV-2), since the mixed antisera recognized viral polypeptides with dif...

journal_title：Journal of virological methods

authors： Okazaki K,Chiya S,Ideguchi S,Kimura S

更新日期：1987-11-01 00:00:00

abstract：:The sensitivity and specificity of four modes of two assays, immediate early nuclear antigen detection in cell culture (IENAD) at 24 and 48 h post-infection (p.i.) by immunofluorescence using a murine monoclonal antibody, and dot-blot DNA hybridisation with overnight or prolonged autoradiography using the 32P-labelled...

journal_title：Journal of virological methods

authors： Morris DJ,Lomax J,Fox AJ,Corbitt G

更新日期：1987-10-01 00:00:00

abstract：:Hexon is the major structural protein of adenovirus, and has significance in studies of virus structure and function, vaccine development, and immunodiagnosis. We describe a simple, single-step, anion-exchange high performance liquid chromatography (HPLC) method for the high yield purification of hexon. Purity of the ...

journal_title：Journal of virological methods

authors： Siegel SA,Hutchins JE,Witt DJ

更新日期：1987-09-01 00:00:00

abstract：:An enzyme-immunoassay (EIA) for antibodies to hepatitis B core antigen (anti-HBc) was developed. The new test uses undiluted samples, incubated directly into an HBcAg coated well. Three alternative test procedures are possible. The stability of reagents was studied and a preclinical evaluation was performed intramural...

journal_title：Journal of virological methods

authors： Matthyssen L,Arndt-Hanser A,Lange W,Maass G,Schütt K,van Loon A,Wolters G

更新日期：1987-08-01 00:00:00

abstract：:A simple one-step ELISA to detect anti-CMV antibodies was developed. The test was based on the inhibition principle, and used anti-CMV coated microtitre plates, CMV nuclear antigen and anti-CMV Fab'-HRP conjugate; total assay time was 1.5 h. The use of Fab'-HRP conjugates improved discrimination between positive and n...

journal_title：Journal of virological methods

authors： Wielaard F,Scherders J,Dagelinckx C,Gausset P

更新日期：1987-08-01 00:00:00

abstract：:This report describes the first time entire viral capsids have been purified using fast protein liquid chromatography (FPLC) techniques. The FPLC is used here to separate the two electrophoretic forms of cowpea mosaic virus. The capsid forms are shown to be separated by the Mono-Q column without damaging the capsids. ...

journal_title：Journal of virological methods

authors： Smith TJ

更新日期：1987-07-01 00:00:00

abstract：:In this inhibition immunoassay undiluted serum reacts in solution with crude cellular CMV antigen in wells of microtestplates coated with hyperimmune CMV-reactive monkey IgG. CMV antibodies in the serum under test block (completely or partial) the fixation of antigen to the capture layer. Unblocked antigenic activity ...

journal_title：Journal of virological methods

authors： Nielsen CM,Hansen K,Andersen HM,Gerstoft J,Vestergaard BF

更新日期：1987-06-01 00:00:00

abstract：:Photobiotin was used to prepare biotinylated, nonradioactive nucleic acid probes for the detection of the RNA of barley yellow dwarf virus (BYDV) in plant extracts. A 1.7-kb cDNA of the PAV isolate of BYDV in the plasmid pUC8 vector was biotinylated and then used intact or as sonicated double-stranded DNA fragments. S...

journal_title：Journal of virological methods

authors： Habili N,McInnes JL,Symons RH

更新日期：1987-06-01 00:00:00

abstract：:Rapid detection of CMV-DNA in urine specimens by dot-blot hybridization was compared to conventional virus isolation and to virus identification using solid-phase immunoelectron microscopy (SPIEM). To detect viral DNA, 32P-labeled EcoR1 J fragment of CMV-DNA was used as a probe in the hybridization assay. In addition,...

journal_title：Journal of virological methods

authors： Vonsover A,Gotlieb-Stematsky T,Sayar Y,Bardov L,Manor Y,Siegal B

更新日期：1987-05-01 00:00:00

abstract：:Immunoblotting ('Western blotting') is routinely used for detection of antibodies against HIV in the diagnosis of HIV infection. We describe an improved procedure, which does not require virus purification and is easy to control for 'false-positive' results. The technique also does not produce erroneous results due to...

journal_title：Journal of virological methods

authors： Thorpe R,Brasher MD,Bird CR,Garrett AJ,Jacobs JP,Minor PD,Schild GC

更新日期：1987-05-01 00:00:00

abstract：:Hyperimmune rat antisera prepared against 5 recent antigenic variants of influenza A (H3N2) viruses were studied for haemagglutination inhibiting (HI) antibodies to the homologous and the heterologous viruses. The ratios of homologous to heterologous reactions varied from one animal to another in immunizations with ea...

journal_title：Journal of virological methods

authors： Pyhälä R,Pyhälä L

更新日期：1987-03-01 00:00:00

abstract：:Ten plasmids containing viral cDNA inserts of portions of the dengue virus type 2 (DEN-2) or Kunjin virus (KUN) genomes were biotinylated using photobiotin acetate and used as probes for the detection of flavivirus RNA in infected Vero cells. The viral cDNA inserts ranged in length from 0.19 to 2.7 kilobase pairs, and...

journal_title：Journal of virological methods

authors： Khan AM,Wright PJ

更新日期：1987-02-01 00:00:00

abstract：:A new technique of alkaline phosphatase amplification in an ELISA (amplified ELISA) was used to increase the sensitivity of detection of barley yellow dwarf virus (BYDV) from oat plant sap and in individual vector aphids. Amplified ELISA differs from conventional direct double antibody sandwich ELISA (DAS-ELISA) in th...

journal_title：Journal of virological methods

authors： Torrance L

更新日期：1987-02-01 00:00:00

abstract：:Conditions for using slot-blot nucleic acid hybridization to quantitatively detect dengue-2 virus using a radiolabelled cDNA probe, pVV17, were determined. As little as 11 plaque-forming units of virus were detected using a hybridization mixture without formamide and performing the test at 70 degrees C. While predomin...

journal_title：Journal of virological methods

authors： Henchal EA,Narupiti S,Feighny R,Padmanabhan R,Vakharia V

更新日期：1987-02-01 00:00:00

abstract：:Immunosorbent electron microscopy (ISEM) and immuno-gold staining (IGS) electron microscopy methods have been applied to human faeces, shown by direct electron microscopy (EM) to contain calicivirus. Caliciviruses were successfully trapped on grids coated with positive rabbit or human antisera against calicivirus, but...

journal_title：Journal of virological methods

authors： Kjeldsberg E

更新日期：1986-11-01 00:00:00

abstract：:Rapid characterization of herpesvirus isolates exemplified by equine herpesviruses is described. Total DNAs were isolated from virus infected small scale cell cultures. The DNA fragments obtained after restriction enzyme digestion were separated on agarose gels, transferred and immobilized on filter membranes. A radio...

journal_title：Journal of virological methods

authors： Chowdhury SI,Hammerschmidt W,Ludwig H,Thein P,Buhk HJ

更新日期：1986-11-01 00:00:00

abstract：:This communication describes the application of Epstein-Barr virus lymphocyte transformation technology to the production of human monoclonal antibodies specific for human cytomegalovirus. A group of such IgG antibodies have been characterized in terms of subclass, light-chain composition, specificity for particular v...

journal_title：Journal of virological methods

authors： Redmond MJ,Leyritz-Wills M,Winger L,Scraba DG

更新日期：1986-08-01 00:00:00

abstract：:Hepatitis B micelles containing the p25 component of hepatitis B surface antigen (HBsAg) have been produced by Triton X-100 solubilization followed by ultracentrifugation in linear sucrose gradients. The product was found to resemble micelle forms prepared from plasma-derived HBsAg with the surface being composed of d...

journal_title：Journal of virological methods

authors： Howard CR,Young PR,Lee S,Dixon J,Zuckerman AJ,McAleer WJ,Lehman ED

更新日期：1986-08-01 00:00:00

abstract：:Two enzyme-linked immunosorbent assays for detection of adenovirus antigen in faecal extracts have been established. A conjugate of rabbit anti-(human) adenovirus immunoglobulin and horseradish peroxidase (HRP) prepared by means of a hetero-bifunctional reagent, N-succinimidyl-3-(2-pyridyldithio) propionate (SPDP), wa...

journal_title：Journal of virological methods

authors： Mortensson-Egnund K,Kjeldsberg E

更新日期：1986-08-01 00:00:00

abstract：:Equine infectious anaemia virus (EIAV) was adapted to the Cf2Th cell line, a heterologous malignant line from canine thymus. A persistent infection was monitored for 100 serial passages by demonstrating the presence of virus and viral antigens at each 10th passage by electron-microscopy, immunodiffusion and immunofluo...

journal_title：Journal of virological methods

authors： Bouillant AM,Nielsen K,Ruckerbauer GM,Samagh BS,Hare WC

更新日期：1986-07-01 00:00:00

abstract：:Biotin-conjugated antisera to two strains of sugarcane mosaic virus and erysimum latent virus were used to detect the viruses in extracts of infected plants. Two methods, enzyme-linked immunosorbent assays and electroblot immunoassays, were used. The antisera were found to be sufficiently sensitive for detection of th...

journal_title：Journal of virological methods

authors： Hewish DR,Shukla DD,Gough KH

更新日期：1986-04-01 00:00:00

abstract：:The simple spot hybridization technique to detect HBV DNA in serum was modified to concentrate Dane particles by pelleting. This minimised interference by serum components and allowed larger volumes of sera (up to 500 microliters) to be used in serial dilution on borderline positive samples and increased the efficienc...

journal_title：Journal of virological methods

authors： Fagan EA,Guarner P,Perera SD,Trowbridge R,Rolando N,Davison F,Williams R

更新日期：1985-12-01 00:00:00

abstract：:A quantitative and simple indirect radioimmunoassay (IRIA) was developed for porcine parvovirus (PPV), employing a monoclonal antibody directed against PPV adsorbed to nitrocellulose membrane. The IRIA was equally sensitive to live or inactivated PPV. There was a linear relationship between membrane-bound radioactivit...

journal_title：Journal of virological methods

authors： Katz JB,van Deusen RA

更新日期：1985-12-01 00:00:00

abstract：:The fluorescent antibody (FA) test for Epstein-Barr virus (EBV)-specific IgM antibody was improved by the use of sodium butyrate to induce a higher level of EBV antigen expression in P3HR-1 slide preparations and by removal of rheumatoid factor (RF) and IgG antibodies from test sera by means of adsorption with suspens...

journal_title：Journal of virological methods

authors： Harnett GB,Palmer CA,Bucens MR

更新日期：1985-10-01 00:00:00

abstract：:A rapid rate-kinetic turbidometric assay for the quantitation of viral complement fixing antibodies has been developed, using adenovirus as a model. The procedure is based on the turbidometric quantitation of intact sheep erythrocytes and measures the rate of hemolysis (change in absorbance at 640 nm/min/), at maximum...

journal_title：Journal of virological methods

authors： Fulton RE,Dininno VL

更新日期：1985-10-01 00:00:00